西伯利亚鲟致病性嗜水气单胞菌外膜蛋白及其抗原性分析

采用十二烷基肌氨酸钠(Sarkosyl)法提取西伯利亚鲟嗜水气单胞菌(Aeromonas hydrophila)外膜蛋白,电泳显示所提取的主要外膜蛋白分子量为26～120 kDa;为比较该菌株与气单胞菌菌属其他细菌外膜蛋白组分及抗原性异同,以致病性豚鼠气单胞菌(A.caviae)、温和气单胞菌(A.sobria)和无致病力的嗜水气单胞菌为对照,电泳图谱显示4种气单胞菌外膜蛋白的分子量主要集中在26～120 kDa之间;利用抗西伯利亚鲟嗜水气单胞菌血清的免疫印迹试验表明该菌株外膜蛋白中分子量为75 kDa、52 kDa、43 kDa、40 kDa、34 kDa、28 kDa的蛋白条带呈现阳性反应,其他3种气单胞菌外膜蛋白中均有与该抗血清反应的条带,且分子量为28 kDa、34 kDa的反应条带为4株菌共有;43 kDa与75 kDa反应条带为部分菌株共有.为进一步筛选和研究致病性气单胞菌的共同保护抗原提供参考.

Antigenicity of outer membrane proteins of pathogenic Aeromonas hydrophila isolated from Siberian sturgeon Acipenser baerii

A pathogenic strain Aeromonas hydrophila was isolated from Siberian sturgeon(Acipenser baerii) suffering with bacterial septicaemia.In this experiment,OMPs of four strains of Aeromonas were obtained by Sarkosyl method combining super centrifugal purification.SDS-PAGE showed that the strain had 14 membrane proteins with molecular weights of from 26 kDa to 120 kDa.OMPs of the pathogencial and unpathogencial Aeromonas hydrophila,Aeromonas caviae and Aeromonas sobria were analyzed and compared by using SDS-PAGE.The results showed that molecular weights focused on from 26 kDa to 120 kDa.After SDS-PAGE,in the Western-blotting with rabbit antiserum against Aeromonas hydrophila isolated from Siberian sturgeon,only 6 OMPs of the strain with molecular weights at 75 kDa,52 kDa,43 kDa,40 kDa,34 kDa and 28kDa were detected.It showed that the 6 OMPs had some antigenicity.And the other 3 strains of Aeromonas all had positive reaction.The band of 28kDa and 34kDa had visible positive reaction in all 4 Aeromonas strains,the band of 43kDa and 75 kDa had positive reaction in some Aeromonas strains.