人工培殖冬虫夏草D-甘露醇的含量测定

采用水提回流离心的方法,提取分离人工培殖冬虫夏草中D-甘露醇,用高碘酸钠与甘露醇反应产生黄色的3,5-二乙酰-1,4-脱氢二甲基吡啶,在波长412 nm处测定其最大吸收.甘露醇浓度在10～50 mg/L之间时,与吸光值(OD412)有良好线性关系,线性方程为C=79.172x-0.0713,相关系数r=0.9999.人工培殖冬虫夏草中D-甘露醇的含量分别为89.64 mg/g(2005年)、91.86 mg/g(2006年)、94.71 mg/g(2007年)、98.98 mg/g(2008年)、97.50 mg/g(2009年)、94.71 mg/g(野生)和96.84 mg/g(野生).人工培殖冬虫夏草与野生冬虫夏草D-甘露醇的含量基本相同.

Determination of D-mannitol in cultivated Cordyceps sinensis

To provide a basis for medicinal value of cultivated Cordyceps sinensis,mannitol was separated by water extraction and centrifugation.The compound(3,5-diacetyl-1,4-dehydro-dimethylpyridine) obtained by the reaction of NaIO4 and C6H12O6 had the largest absorption at wave length of 412 nm.The absorbance values(OD412 value) and mannitol concentration showed a good linearity at the range of 10-50 mg/L.The linear equation is C= 79.172x-0.0713(r=0.9999).The contents of mannitol in cultivated Cordyceps sinensis were 89.64 mg/g(2005a,cultivated),91.86 mg/g(2006a,cultivated),94.71 mg/g(2007a,cultivated),98.98 mg/g(2008a,cultivated),97.50 mg/g(2009a,cultivated),94.71 mg/g(2008a,wild) and 96.84 mg/g(2008a,wild),respectively.The results showed the mannitol content of the cultivated Cordyceps sinensis was equal roughly to the wild Cordyceps sinensis.