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| 群体感应抑制活性海洋细菌的筛选与鉴定 | |
| 引用本文： | 于艳杰,李庆铭,杨丽庆,梁景霞,孙其富,朱虎.群体感应抑制活性海洋细菌的筛选与鉴定[J].生物学杂志,2012,29(2):24-26. |
| 作者姓名： | 于艳杰 李庆铭 杨丽庆 梁景霞 孙其富 朱虎 |
| 作者单位： | 1. 中国石油大学,华东,生物工程与技术中心,青岛266555 2. 山东省济宁市生物化学工业办公室,济宁,272017 3. 山东省胶南市农业局,山东,胶南266400 |
| 基金项目： | 中央高校基本科研业务费专项资金项目，国家大学生创新性实验计划项目，山东省自然科学基金 |
| 摘 要： | 从黄海青岛海域海洋污泥中分离纯化微生物菌株,然后采用紫色色杆菌(Chromobacterium violaceum) 为指示菌株,检测其代谢产物的群体感应(Quorum sensing) 抑制活性,并对具有抑制功能的细菌菌株进行16S rDNA分子鉴定及生理生化特征分析.结果表明,1株蜡样芽孢杆菌和1株水莱茵海默氏菌具有较强的细菌群体感应抑制活性,分别命名为Bacillus cereus QSI01和Rheinheimera aquimaris QSI02.从海洋环境中筛选的具有细菌群体感应抑制活性的菌株,为以致病菌群体感应系统为靶点的新型药物的研发提供了菌种资源. |
| 关 键 词： | 细菌群体感应 海洋细菌 蜡样芽孢杆菌 水莱茵海默氏菌 抑制剂 |
Isolation and identification of marine bacterium with capability of quorum sensing quenching |
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| YU Yan-jie , LI Qing-ming , YANG Li-qing , LIANG Jing-xia , SUN Qi-fu , ZHU Hu.Isolation and identification of marine bacterium with capability of quorum sensing quenching[J].Journal of Biology,2012,29(2):24-26. | |
| Authors: | YU Yan-jie LI Qing-ming YANG Li-qing LIANG Jing-xia SUN Qi-fu ZHU Hu |
| Institution: | 1(1.Center for Bioengineering and Biotechnology,China University of Petroleum(East China),Qingdao 266555; 2.Office of Jining Biochemical Industry,Jining 272017;3.Jiaonan Agriculture Bureau,Jiaonan 266400,China) |
| Abstract: | Marine bacterial strains were isolated from the sludge of Yellow Sea,and their extracts were detected using Chromobacterium violaceum as indicator strains.The extracts of Bacillus cereus QSI01 and Rheinheimera aquimaris QSI02 had QS inhibitory activity.The research solved the critical technical problems of screening bioactive metabolites,and provided the key parameters for industrialization study of production of bioactive substances from ocean sources.Result will be used for sustainable developments of new natural antibacterial compounds. |
| Keywords: | bacterial quorum sensing marine bacterium Bacillus cereus Rheinheimera aquimaris quorum-quenching |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |