Polynucleotide phosphorylase covalently bound to cellulose and its use in the preparation of homopolynucleotides |
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| Authors: | C H Hoffman E Harris S Chodroff S Michelson J W Rothrock E Peterson W Reuter |
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| Affiliation: | 1. Beijing Key Laboratory of Urban Hydrological Cycle and Sponge City Technology, College of Water Sciences, Beijing Normal University, Beijing 100875, PR China;2. Department of Physiological Sciences and Center for Environmental and Human Toxicology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA;3. University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, USA;4. State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, School of Environment, Northeast Normal University, Changchun, Jilin 130117, PR China |
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| Abstract: | A readily prepared, crude concentrate of polynucleotide phosphorylase (PNPase) has been covalently bound by the method of Porath to cyanogen bromide-activated cellulose; this is the first report of a synthesizing enzyme bound to a solid support. The bound enzyme had good activity and stability; more than forty consecutive polymerization cycles of nucleoside diphosphates have been carried out with a single preparation to give consistently good yields of stable polymers, and these were readily separated from the bound phosphorylase without tedious phenol extractions. |
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