胡萝卜愈伤组织形成过程中激素和创伤诱导的钙调素水平的变化

CaM抑制剂TFP抑制了胡萝卜愈伤组织的形成。ELISA法测定CaM的动态表明,愈伤组织形成过程中,出现了两个CaM 的峰。第一个峰值在6h,可能是由创伤引起的,单位蛋白和单位鲜重的CaM 量都增加了1倍左右,第二个峰值可能是由激素引起的,从48 h以后开始增加,7天时单位鲜重的CaM 增加了4倍左右,单位蛋白的CaM 量也特异地增加了0.7倍,此结果亦为CaM 免疫荧光组织化学定位结果所证实。研究结果表明胡萝卜愈伤组织形成过程中激素与创伤效应和CaM 动态有关。

CHANGES OF CALMODULIN LEVELS INDUCED BY PHYTOHORMONE AND WOUNDS DURING CARROT CALLUS FORMATION

The CaM antagonist,Trifluoperizine(TFP),was found to inhibit carrot callusformation,and the inhibion could be partlyrecovered by removing the antagonist.Ac-cording to the ELISA analyses,the varyingCaM levels induced by phytohormone andwounds formed two peaks during the callusformation.The first peak,with CaM leveldoubled,appeared at the 6 th hour in cul-ture,and may be considered as the effectof wounds for it made no difference whe-ther phytohormone present or not.Thesecond peak began at the 48 th hour in cul-ture and reached its maximum,with CaMlevel four times higher,on the 7 th day,meanwhile the callus began to appear onthe 5 th day in culture.The CaM localiza-tion experiments by immunofluoresence me-thod also revealed that CaM levels gradua-lly increased in explants along with cultu-red time.The explants cultured free fromphytohormone did not show the second peakand could not form callus at last,whichimplies the second peak was induced bythe phytohormone.Actinomycin D and cy-cloheximide both inhibited the second peakwhile neither affected the first one.All theseresults suggested that CaM may play animportant role in the effects of phytohor-mone and wounds during carrot callus for-mation.