Simplified method of making alginate-polylysine microcapsules for hybridoma cell culture using RPMI 1640 medium |
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| Authors: | Gyun Min Lee Bernhard O. Palsson |
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| Affiliation: | (1) Department of Chemical Engineering, University of Michigan, 48109 Ann Arbor, MI, USA |
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| Abstract: | The method of making alginate-poly-L-lysine microcapsules for hybridoma cell culture can be simplified by cultivating the cells in RPMI 1640 medium. Phosphate concentration in RPMI 1640 medium is sufficiently high to dissolve the alginate gel and, thereby, can be used to eliminate the step of citrate buffer treatment required for reliquefying the interior alginate gel. |
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