A new high-performance liquid chromatography assay for glutamine synthetase and glutamate synthase in plant tissues |
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| Authors: | F Martin A Suzuki B Hirel |
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| Institution: | 1. Unité de Microbiologie des Sols, INRA-CNRF, Champenoux, 54 280 Seichamps, France;2. Laboratoire de Physiologie Cellulaire Végétale, ERA au CNRS 799, Centre d''Orsay, Université de Paris-Sud, 91 405 Orsay, Cédex, France |
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| Abstract: | Glutamic acid and glutamine, formed in plant tissue extracts by glutamate synthase and glutamine synthetase, respectively, were separated by derivatization with o-phthaldialdehyde followed by reverse-phase high-performance liquid chromatography on a μBondapak C18 column. The derivatives were eluted by isocratic elution, and the mobile phase was a 20 mm sodium phosphate buffer (pH 6.8) with 36% methanol. The procedure is rapid, sensitive, and requires a minimum sample. |
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