τ and Microtubule-Associated Protein 2c Transfection and Neurite Outgrowth in ND 7/23 Cells |
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Authors: | Anja Langkopf Jocelyne Guilleminot Jacques Nunez |
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Affiliation: | UnitéINSERM 282 affiliée au CNRS, Hôpital Henri Mondor, Créteil, France |
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Abstract: | Abstract: Neuronal hybrid ND 7/23 cells, which display sensorylike properties, develop neurites when cultured in the presence of either dibutyryl cyclic AMP plus nerve growth factor (DBcAMP + NGF) or retinoic acid or a phorbol ester derivative, although they express only trace amounts of the microtubule-associated τ proteins and low levels of microtubule-associated protein 2c (MAP2c). Nondifferentiated ND cells transfected with τ cDNAs did not develop neurites, whereas very short cell processes were formed in MAP2c-transfected cells. τ and MAP2 antibodies labeled microtubule bundles displayed in a ring array underneath the surface of the transfected cells and short microtubules starting from the cell center. After differentiation in the presence of DBcAMP + NGF, the same bundle organization was observed in the transfected cells. In addition, τ and MAP2 antibodies stained a short section of the formed neurites. These data demonstrate that the expression of τ protein is not sufficient to induce neurite extension and that other proteins induced by morphogens are more important to initiate morphological differentiation of this cell line. |
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Keywords: | Neurons Differentiation Microtubules τ Microtubule-associated protein 2c Transfection |
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