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Sarkosyl is a good regeneration reagent for studies on vacuolar-type ATPase subunit interactions in Biacore experiments |
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| Authors: | Rahman Suhaila Arai Satoshi Saijo Shinya Yamato Ichiro Murata Takeshi |
| Institution: | aDepartment of Biological Science and Technology, Tokyo University of Science, Noda-shi, Chiba 278-8510, Japan;bDepartment of Chemistry, Graduate School of Science, Chiba University, Inage, Chiba 263-8522, Japan;cRIKEN SPring-8 Center, Sayo, Hyogo 679-5148, Japan;dRIKEN Systems and Structural Biology Center, Tsurumi, Yokohama 230-0045, Japan |
| Abstract: | Biacore is widely used for studies on protein–protein interaction in which regeneration is one of the most important steps. Here we introduce the anionic detergent sodium lauroyl sarcosinate (sarkosyl), which works satisfactorily as a regeneration reagent. After regeneration by the mild detergent, the subsequent binding experiment was reproducible without any degradation of the ligand. This regeneration condition can be employed for diverse combinations of ligand–analyte binding interactions and optimized as required. |
| Keywords: | Surface plasmon resonance Regeneration Sarkosyl Biacore V-ATPase |
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