|
|||||
|
|
Antigenic immunostaining patterns in somatic hybrids of human HeLa cells and mouse fibroblasts 3T3.4E propagated in conventional medium and delipidized serum |
|
| Authors: | T Gallagher Y Chardonnet J Viac J Patet A Lefèvre |
| Institution: | 1. INSERM U 209CNRS UA 601, Clinique Dermatologique (Pr J. Thivolet) Pav R, H?pital E. Herriot, 69437, Lyon Cedex 03, France 2. Long Island Jewish Medical Center, 11040, New Hyde Park, NY, USA 3. INSERM U 51, CNRS UA 609 Unité de Virologie, Institut Mérieux, 1 Place Pr J. Renaut, 69008, Lyon Cedex, France 4. INSERM U 293, Ecole Normale Supérieure, 1 Rue Maurice Arnoux, 92120, Montrouge, France |
| Abstract: | Somatic cell hybrids were obtained with electric pulse by fusion of human epithelial HeLa cells derived from a carcinoma of the uterine cervix and mouse fibroblasts 3T3.4E, deficient in thymidine kinase. Hybrids were selected and propagated in HAT media; some experiments were carried out in medium with delipidized serum. The hybrid cells were characterized by indirect immunofluorescence with a biotin-streptavidin system using a panel of nine monoclonal antibodies specific for membrane and cytoplasmic antigens of parental cells: intermediate filaments (keratins and vimentin), HLA class 1 (β2-microglobulin), cell activation (EGF and transferrin receptors) and cellular adhesion (fibronectin and laminin). |
| Keywords: | Human HeLa/murine fibroblast hybrids Immunostaining Conventional medium Delipidized serum |
| 本文献已被 SpringerLink 等数据库收录！ | |