| 先锋牧草-香根草联合固氮菌多样性研究 |
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| 引用本文： | 赵现伟,Chaudhary Hassan Jave,何玉梅,张志英,彭桂香,谭志远. 先锋牧草-香根草联合固氮菌多样性研究[J]. 微生物学报, 2009, 49(11): 1430-1437 |
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| 作者姓名： | 赵现伟 Chaudhary Hassan Jave 何玉梅 张志英 彭桂香 谭志远 |
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| 作者单位： | 广东省植物分子育种重点实验室，农学院，华南农业大学；2资源环境学院，华南农业大学，广州510642;广东省植物分子育种重点实验室，农学院，华南农业大学；2资源环境学院，华南农业大学，广州510642;广东省植物分子育种重点实验室，农学院，华南农业大学；2资源环境学院，华南农业大学，广州510642;广东省植物分子育种重点实验室，农学院，华南农业大学；2资源环境学院，华南农业大学，广州510642;广东省植物分子育种重点实验室，农学院，华南农业大学；2资源环境学院，华南农业大学，广州510642;广东省植物分子育种重点实验室，农学院，华南农业大学；2资源环境学院，华南农业大学，广州510642 |
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| 基金项目： | 国家自然科学基金（30470002，30770001）；教育部新世纪优秀人才支持项目(NCET-07-0315)；广东省农业攻关项目(2007B020711004) |
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| 摘 要： | 摘要：【目的】香根草（Vetiver zizanioides）是一种多年生禾本科草本植物，具有极强的生态适应性和抗逆能力，可作饲料和水土保持用。通过研究香根草联合固氮菌多样性，为进一步研究和应用打下基础。【方法】采用无氮培养基，首次从香根草中分离到47株联合固氮菌，分别应用SDS-PAGE全细胞蛋白质电泳、DNA指纹图谱、唯一碳源和16S rDNA全序列测定等方法，进行聚类和多样性分析。【结果】SDS-PAGE、IS-PCR和Bio-BIQA碳源利用的聚类结果基本一致，将供试菌株分为6个类群和4个单菌株；
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| 关 键 词： | 关键词：香根草；联合固氮菌；多样性 |
| 收稿时间： | 2009-04-10 |
| 修稿时间： | 2009-08-14 |
Diversity of associated nitrogen-fixing bacteria isolated from the pioneer plants-Vetiver zizanioides |
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| Xianwei Zhao,Chaudhary Hassan Jave,Yumei He,Zhiying Zhang,Guixiang Peng and Zhiyuan Tan. Diversity of associated nitrogen-fixing bacteria isolated from the pioneer plants-Vetiver zizanioides[J]. Acta microbiologica Sinica, 2009, 49(11): 1430-1437 |
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| Authors: | Xianwei Zhao Chaudhary Hassan Jave Yumei He Zhiying Zhang Guixiang Peng Zhiyuan Tan |
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| Affiliation: | Guang Dong Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University; 2College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China;Guang Dong Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University; 2College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China;Guang Dong Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University; 2College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China;Guang Dong Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University; 2College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China;Guang Dong Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University; 2College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China;Guang Dong Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University; 2College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | Abstract: [Objective] Vetiver zizanioides is a perennial grass of the Poaceae family, known as silage, soil and water conservation role. The aim is to collect and identify the resources of the nitrogen-fixing bacteria associated with Vetiver zizanioides. [Methods] Associated nitrogen-fixing bacteria isolated from Vetiver zizanioides, were studied by SDS-PAGE whole-cell protein patterns, insert sequence (IS)-PCR finger printing, utilization of sole carbon sources and 16S rRNA gene sequence analysis. [Results] Based on the results of finger printing analysis, protein patterns and biological test, isolates were grouped into 6 clusters, except 4 single strains. Phylogenetic analysis of 16S rDNA sequences indicated that isolates belonged to Herbaspirillum frisingense, Enterobacter ludwigii, Pseudacidovorax intermedius, Mitsuaria chitosanitabida, Pseudomonas putida, Pseudomonas fluorescens, Burkholderia vietnamiensis and Enterobacter cloacae. [Conclusion] The nitrogen fixers associated with Vetiver zizanioides showed great diversity and may have a potential application for the grass forage and agriculture. |
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| Keywords: | Key words: Vetiver zizanioides associated nitrogen-fixing bacteria diversity |
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