Determination of SRS-A release from guinea-pig lungs by a radioimmunoassay |
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| Authors: | D Aharony P Dobson P R Bernstein E J Kusner R D Krell J B Smith |
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| Affiliation: | 1. Pulmonary Pharmacology, Biomedical Research Department, Stuart Pharmaceuticals, Division of ICI Americas Inc., Wilmington, DE 19897 USA;2. Medicinal Chemistry Section, Biomedical Research Department, Stuart Pharmaceuticals, Division of ICI Americas Inc., Wilmington, DE 19897 USA;3. Center for Thrombosis Research, Temple University, Philadelphia, PA 19140 USA |
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| Abstract: | A sensitive radioimmunoassay for leukotrienes (LTs) has been developed. Rabbits were immunized with a conjugate of LTD4 and bovine serum albumin, prepared by using 1,5-difluoro-2,4-dinitrobenzene as the coupling agent. The assay can detect 0.045 pmol LTD4 at a final plasma dilution of 1:72. 50% displacement of bound 3H-LTD4 was obtained with 0.43 +/- 0.03 pmol LTD4. LTC4, LTE4 and LTF4 cross-react 159%, 57% and 85%, respectively, whereas LTB4, 5-HETE and prostaglandins did not. The assay was validated by measuring the antigen-induced release of LTs from sensitized guinea-pig chopped lungs. High correlation (0.9434, p less than 0.05) was found when LTs were simultaneously determined by this assay and a bioassay on guinea pig ileum. |
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