Production of high-content inulo-oligosaccharides from inulin by a purified endoinulinase |
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| Authors: | J.W. Yun D.H. Kim T.B. Uhm S.K. Song |
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| Affiliation: | (1) Dept. of Biotechnology, Taegu University, Kyungbuk, 712-714, Korea;(2) Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University, Chonbuk, 561-756;(3) Dept. of Chemical Engineering, Pusan National University, Pusan, 609-735, Korea |
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| Abstract: | Inulo-oligosaccharides were produced from inulin with high yield by using a purified endoinulinase from a commercial inulinase preparation. The maximum yield of oligosaccharide achieved was around 96% irrespective of substrate concentrations ranged from 50 to 150 g inulin/l. A wide range of degradation products from inulin, varying in their DP (degree of polymerization) 2 to 6, were obtained where the major oligosaccharides were DP3 and 4. The reaction pH gave rise to a significant difference in yield and sugar composition of inulo-oligosaccharides. |
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