Microfluidic technologies for studying synthetic circuits |
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Authors: | Lin Benjamin Levchenko Andre |
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Institution: | Department of Biomedical Engineering, Institue for Cell Engineering, Johns Hopkins University, 3400 North Charles Street, Clark Hall room 207, Baltimore, MD 21218, USA. |
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Abstract: | Advances in synthetic biology have augmented the available toolkit of biomolecular modules, allowing engineering and manipulation of signaling in a variety of organisms, ranging in complexity from single bacteria and eukaryotic cells to multi-cellular systems. The richness of synthetic circuit outputs can be dramatically enhanced by sophisticated environmental control systems designed to precisely pattern spatial-temporally heterogeneous environmental stimuli controlling these circuits. Moreover, the performance of the synthetic modules and 'blocks' needed to assemble more complicated networks requires more complete characterization as a function of arbitrarily complex environmental inputs. Microfluidic technologies are poised to meet these needs through a variety of innovative designs capitalizing on the unique benefits of manipulating fluids on the micro-scales and nano-scales. This review discusses the utility of microfluidics for the study of synthetic circuits and highlights recent work in the area. |
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