Ethylene inhibitors promote in vitro regeneration of cowpea (Vigna unguiculata L.) |
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| Authors: | Mohanjeet S Brar Misty J Moore Jameel M Al-Khayri Teddy E Morelock Edwin J Anderson |
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| Institution: | (1) Department of Plant Pathology, University of Arkansas, 72701 Fayetteville, Arkansas;(2) Department of Horticulture, University of Arkansas, 72701 Fayetteville, Arkansas;(3) Date Palm Research Center, King Faisal University, P.O. Box 400, 31982 Al-Hassa, Saudi Arabia |
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| Abstract: | Summary Ethylene is a plant growth regulator that is known to influence in vitro morphogenesis. This study investigated the effects
of three ethylene inhibitors, silver nitrate (AgNO3), 2,5-norbornadiene, and cobalt chloride (CoCl2), on the regeneration of cowpea from cotyledon explants. Significant increases in the percentage of regeneration occurred
as a result of adding either 50 μM AgNO3 or 100 μM 2,5-norbornadiene. The number of shoots produced per explant was enhanced by adding 25 μM CoCl2 or 100 μM norbornadiene. Maximum shoot elongation was obtained with 25 μM of either CoCl2 or norbornadiene. The effect of the duration of exposure to AgNO3 was also determined. The greatest percent regeneration was obtained with the addition of 60 μM AgNO3 either to both the initiation and regeneration stages, or to only the regeneration stage. The promotive effects on organogenesis
in response to ethylene inhibitors suggests an important role for ethylene in the process of in vitro morphogenesis of cowpea
and may contribute to its normally low regeneration frequency. |
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| Keywords: | southernpea micropropagation tissue culture silver nitrate 2 5-norbornadiene cobalt chloride |
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