Studies on plant tissue cultures I. Relationship between inocula sizes and growth of calluses in liquid culture |
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Authors: | SYONO, KUNIHIKO FURUYA, TSUTOMU |
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Affiliation: | College of Pharmaceutical Sciences, Kitasato University Shiba, Minato-ku, Tokyo |
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Abstract: | - 1. It was observed that lag of growth was longer in small inoculathan in large inocula using tobacco callus in liquid culture.
- 2. These different growth responses between small and largeinocula were dependent on the ratio of inoculum to culture medium.
- 3. The same result was obtained in a strain of carrot rootcallus.But the growth lag was very short in the carrot callus,whichwas subcultured for the shortest period among the 4 strainsused, even in small inocula. On the other hand, both small andlarge inocula of the strain, which were subcultured for thelongest period among the 4 strains, did not grow at all duringthe culture period; the longer the period of subculturing, thelonger the lag of growth.
- 4. The longer lag of small inoculain tobacco callus was recoveredby gibberellin A3 in the presenceof the acidic fraction ofcarrot root extract or vitamins suchas pyridoxine and thiamine.
(Received December 11, 1967; ) |
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