Novel baculovirus expression vectors that provide sialylation of recombinant glycoproteins in lepidopteran insect cells |
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Authors: | Jarvis D L Howe D Aumiller J J |
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Institution: | Department of Molecular Biology, University of Wyoming, Laramie, WY 82071-3944, USA. DLJarvis@uwyo.edu |
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Abstract: | This report describes novel baculovirus vectors designed to express mammalian beta1,4-galactosyltransferase and alpha2,6-sialyltransferase genes at early times after infection. Sf9 cells infected with these viral vectors, unlike cells infected with a wild-type baculovirus, produced a sialylated viral glycoprotein during the late phase of infection. Thus, the two mammalian glycosyltransferases encoded by these viral vectors are necessary and sufficient for sialylation of a foreign glycoprotein in insect cells under the conditions used in this study. While some of the new baculovirus vectors described in this study produced less, one produced wild-type levels of infectious budded virus progeny. |
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