Effect of UV-C irradiation on stilbene synthase localization in young grape plants |
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| Authors: | K Tang F Fang H R Yang W D Huang |
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| Institution: | 1.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing,China;2.Institute of Agro-Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing,China |
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| Abstract: | Stilbene synthase (STS) is a pivotal enzyme that catalyzes the biosynthesis of resveratrol and could be induced by UV-C irradiation.
However, at present the effect of UV-C irradiation on tissue and subcellular localization of STS is not studied. In this work,
subcellular localization of STS in young grape (Vitis vinifera L. cv. Cabernet Sauvignon) plants exposed to UV-C was examined immunohistochemically using a polyclonal antibody raised against
grape berry STS. The immunohistochemical analysis showed that the UV-induced STS occurred in palisade tissues of grape leaves
and phloem tissues of grape leaf veins, stems, and roots. At the subcellular level, the enhanced STS stimulated by UV-C light
was visualized in the cell walls, chloroplasts (plastids), cytoplasm, and nucleus of the phloem (stems and roots), while only
in chloroplasts in mesophyll cells. This distribution pattern of STS arising in response to UV-C irradiation may be closely
involved in its defense function, which needs much more in-depth evidence to confirm. |
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