New proteins orthologous to cerato-platanin in various Ceratocystis species and the purification and characterization of cerato-populin from Ceratocystis populicola |
| |
Authors: | Cecilia Comparini Lara Carresi Eleonora Pagni Francesca Sbrana Federico Sebastiani Nicola Luchi Alberto Santini Paolo Capretti Bruno Tiribilli Luigia Pazzagli Gianni Cappugi Aniello Scala |
| |
Affiliation: | (1) Dipartimento di Biotecnologie Agrarie, Sezione di Patologia Vegetale, Università di Firenze, Via della Lastruccia 10, 50019 Sesto Fiorentino, Italy;(2) Dipartimento di Scienze Biochimiche, Università di Firenze, Viale Morgagni 50, 50134 Firenze, Italy;(3) Istituto per la Protezione delle Piante del CNR, Sezione di Firenze, Via Madonna del Piano, Palazzo E, 50019 Sesto Fiorentino, Italy;(4) C.S.D.C, Dip. Fisica, Università di Firenze, Via Sansone 1, 50019 Sesto Fiorentino, Italy |
| |
Abstract: | Natural variants of cerato-platanin (CP), a pathogen associated molecular pattern (PAMP) protein produced by Ceratocystis platani (the causal agent of the plane canker stain), have been found to be produced by other four species of the genus Ceratocystis, including five clones of Ceratocystis fimbriata isolated from different hosts. All these fungal strains were known to be pathogenic to plants with considerable importance in agriculture, forestry, and as ornamental plants. The putative premature proteins were deduced on the basis of the nucleotide sequence of genes orthologous to the cp gene of C. platani; the deduced premature proteins of Ceratocystis populicola and Ceratocystis variospora reduced the total identity of all the others from 87.3% to 60.3%. Cerato-populin (Pop1), the CP-orthologous protein produced by C. populicola, was purified and characterized. Pop1 was a well-structured α/β protein with a different percentage of the α-helix than CP, and it self-assembled in vitro in ordered aggregates. Moreover, Pop1 behaved as PAMP, since it stimulated poplar leaf tissues to activate defence responses able to reduce consistently the C. populicola growth. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
Keywords: | Orthologous genes Populus sp. MALDI-TOF Circular dichroism PAMP activity Induction of plant resistance |
本文献已被 SpringerLink 等数据库收录! |
|