Time-dependent inhibition of inositol-1,4,5-trisphosphate-5-phosphatase by calmidazolium chloride in rat GH3 cells. |
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Authors: | Christopher J Fowler and Anne-Marie Eriksson |
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Institution: | Astra Arcus AB, CNS Preclinical R & D, S-151 85, Södertälje, Sweden |
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Abstract: | The calmodulin inhibitor calmidazolium chloride inhibited the activity of soluble and particulate Ins(1,4,5)P3-5-phosphatase from GH3 cells, with an
50 value of 100 μM following a 10-min preincubation with enzyme. The inhibition was time-dependent and could not be reversed by washing of the particulate fraction. It is concluded that although the inhibitory effect of calmidazolium chloride cannot be related per se to inhibition of calmodulin function, effects of this compound unrelated to actions upon calmodulin function may be found when concentrations that are only moderately supramaximal are used. |
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Keywords: | Ins(1 4 5)P3 Ins(1 4 5)P3-5-phosphatase calmidazolium chloride cultured cells |
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