A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids |
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| Authors: | D Wessel U I Flügge |
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| Institution: | Lehrstuhl für Biochemie der Pflanze, Untere Karspüle 2, 3400 Göttingen, Federal Republic of Germany |
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| Abstract: | A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed. The effectiveness of this method is not affected by the presence of detergents, lipids, salt, buffers, and beta-mercaptoethanol. |
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| Keywords: | protein precipitation TX-100 lipids β-mercaptoethanol sodium dodecyl sulfate-polyacrylamide gel electrophoresis |
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