Identification of genes associated with regenerative success of <Emphasis Type="Italic">Xenopus laevis</Emphasis>hindlimbs |
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Authors: | Esther J Pearl Donna Barker Robert C Day Caroline W Beck |
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Institution: | (1) Department of Zoology, University of Otago, P.O. Box 56, 9054 Dunedin, New Zealand;(2) Laboratory of Molecular Organogenesis, Institut de Recherches Cliniques de Montreal (IRCM), 110 avenue des Pins Ouest, QC H2W 1R7 Montreal, Canada;(3) Biochemistry Department, University of Otago, P.O. Box 56, Dunedin, 9054, New Zealand |
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Abstract: | Background Epimorphic regeneration is the process by which complete regeneration of a complex structure such as a limb occurs through
production of a proliferating blastema. This type of regeneration is rare among vertebrates but does occur in the African
clawed frog Xenopus laevis, traditionally a model organism for the study of early development. Xenopus tadpoles can regenerate their tails, limb buds and the lens of the eye, although the ability of the latter two organs to
regenerate diminishes with advancing developmental stage. Using a heat shock inducible transgene that remains silent unless
activated, we have established a stable line of transgenic Xenopus (strain N1) in which the BMP inhibitor Noggin can be over-expressed at any time during development. Activation of this transgene blocks
regeneration of the tail and limb of Xenopus tadpoles. |
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