Effect of TGF-beta 1, TGF-beta 2, and bFGF on chick cartilage and muscle cell differentiation |
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| Authors: | J N Schofield L Wolpert |
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| Affiliation: | Department of Anatomy and Developmental Biology, University College, Middlesex Hospital School of Medicine, London, United Kingdom. |
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| Abstract: | In insulin containing defined medium TGF-beta 1, TGF-beta 2, and bFGF all stimulate chondrogenic differentiation in high-density micromass cultures of distal limb bud mesenchyme cells of chick embryos. In addition bFGF inhibits myogenic differentiation, while TGF-beta 1 and TGF-beta 2 appear to have no effect. TGF-beta 1 and bFGF together act additively to enhance chondrogenesis, while TGF-beta blocks the bFGF inhibitory action on myoblasts, thus allowing them to differentiate. In the absence of insulin, the inhibitory effect of bFGF on muscle cell differentiation is reduced; cartilage differentiation in the presence of the above growth factors is also slightly reduced. |
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