In-situ determination of the P-relations around the primary root of maize with respect to inorganic and phytate-P |
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Authors: | Frank Hübel Erwin Beck |
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Institution: | 1. Scottish Agricultural Statistics Service, MLURI, Craigiebuckler, AB9 2QJ, Aberdeen, UK 2. Macaulay Land Use Research Institute, Craigiebuckler, AB9 2QJ, Aberdeen, UK 3. Scottish Agricultural Statistics Service, University of Edinburgh, EH9 3JZ, Edinburgh, UK
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Abstract: | Autoradiographs of soil slices mapping the distribution of phytate-derived33P around the primary root of 6-day-old maize seedlings were used to investigate the uptake of phytate by the root. Analysis
of the autoradiographs with a laser densitometer and processing of the data with image analysing software resulted in a resolution
of 40 μm. The effect of33P-crossfire was corrected by analysis of the apparent33P-gradient around a phosphate-impermeable teflon tube that was inserted into the labeled soil as a standard. In spite of the
high resolution achieved, a significant depletion zone could not be detected when the soil was equilibrated with33P-phytate. However, with33P-inorganic phosphate, 2 concentric zones were obvious. Within the inner zone, P was accumulated by about 20%, while in the
outer zone a corresponding depletion of P could be detected. The accumulation zone coincided with the extension of the root
hair cylinder, whereas the depleted area was clearly beyond the range of the root hairs. |
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Keywords: | autoradiography freeze-cutting maize P-accumulation P-depletion phytate-P |
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