Intranasal immunization with temperature-sensitive mutants protects granulocytopenic mice from lethal pulmonary challenge withPseudomonas aeruginosa |
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| Authors: | Daniel O Sordelli Veronica E García M Cristina Cerquetti Patricia A Fontán Dr Anne Morris Hooke |
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| Institution: | (1) Department of Microbiology, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina;(2) Center for Pharmacological and Botanical Studies (CEFYBO), Buenos Aires, Argentina;(3) Department of Microbiology, Miami University, 45056 Oxford, Ohio, USA |
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| Abstract: | Intranasal (i.n.) immunization with two temperature-sensitive (ts) mutants ofPseudomonas aeruginosa protected, in a dose-related manner, granulocytopenic (GCP) mice challenged with a lethal dose of the wild-type (wt) organism. The number of ts mutants in oronasopharyngeal lavage fluids and stools decreased steadily in both normal and GCP mice after i.n. immunization. Intranasal immunization with 107 colony-forming units (CFU) of either mutant induced significant protection, whereas intraperitoneal (i.p.) immunization with similar doses induced lower protection. Protection induced by i.n. immunization was accompanied by increased levels of anti-P. aeruginosa IgA in lung lavage fluids. The results of this study demonstrate the usefulness of ts mutants ofP. aeruginosa for local immunization to protect GCP hosts from fatalP. aeruginosa pneumonia. |
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