| Abstract: | Serological performances obtained with five reagents for anti-LAV/HTLVIII antibody screening were compared in individual test and in pool. A panel of 55 selected samples and 2,079 pools prepared from 10,395 unselected samples of blood donors was studied. A first dilution is achieved by pooling, then a second dilution allows to obtain the exact working dilution recommended for the test. The sensitivity in pool method is always similar to the sensitivity in individual test, no false negative was observed. The specificity, evaluated on unselected samples, is slightly inferior to that observed in individual test: false-positive rate is in generally less than 3% compared to 2% average rate routinely observed with individual test. These results allow to propose this method, which save about 75% of the reagent cost, for blood donor screening in all laboratories and especially in developing countries. |
