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Aquaporin-1在大鼠睾丸输出小管的免疫组织化学定位研究
引用本文:张良,黄铁柱,周新华,张泉,王得志.Aquaporin-1在大鼠睾丸输出小管的免疫组织化学定位研究[J].中国组织化学与细胞化学杂志,2003,12(3):291-294.
作者姓名:张良  黄铁柱  周新华  张泉  王得志
作者单位:1. 武汉大学医学院人体解剖学系,武汉,430071
2. 郧阳医学院人体解剖学系,十堰,442000
摘    要:目的研究正常大鼠睾丸输出小管上皮细胞上Aquaporin.1(AQP-1)的定位分布以期了解其在水重吸收上的作用机制。方法对正常wistar大鼠睾丸输出小管进行常规免疫组织化学方法染色观察。结果在睾丸输出小管非纤毛上皮细胞的刷状缘及基侧部AQP-1阳性表达强烈,核上区的胞内体的质膜上也有阳性表达;纤毛上皮细胞的纤毛亦呈阳性反应。结论Aquaporin-1可能与睾丸输出小管非纤毛上皮细胞水重吸收功能有密切关系。

关 键 词:Aquaporin-1  大鼠  睾丸输出小管  水通道蛋白  免疫细胞化学
修稿时间:2003年7月17日

IMMUNOHISTOCHEMICAL LOCALIZATION OF AQUAPORIN-1 IN THE DUCTULI EFFERENTES OF WISTAR RAT
Zhang Liang,Huang Tiezhu,Zhou Xinhua,Zhang Quan,Wang Dezhi.IMMUNOHISTOCHEMICAL LOCALIZATION OF AQUAPORIN-1 IN THE DUCTULI EFFERENTES OF WISTAR RAT[J].Chinese Journal of Histochemistry and Cytochemistry,2003,12(3):291-294.
Authors:Zhang Liang  Huang Tiezhu  Zhou Xinhua  Zhang Quan  Wang Dezhi
Institution:Zhang Liang,Huang Tiezhu*,Zhou Xinhua,Zhang Quan,Wang Dezhi
Abstract:Objective To investigate the cellular expression and localization of Aquaporin-1 (AQP-1) in the efferent ductules of testis.Method Light micrscopy and immunocytochemistry were utilized to localize AQP-1 in the efferent ductules.Result In the efferent ductules, AQP-1 was expressed on the microvilli, basolateral plasma membranes, and apical endosomes of the nonciliated cells and on the cilia of the ciliated cells.Conclusion These results suggest that AQP-1 plays an important role in the fluid reabsorption in the efferent ductules.
Keywords:Aquaporin-1  Immunocytochemistry  Ductuli efferentes  Rat
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