Exopolysaccharide and extracellular metabolite production by Lactobacillus delbrueckii subsp. bulgaricus, grown on lactose in continuous culture |
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Authors: | Alan Welman Ian Maddox Richard Archer |
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Affiliation: | (1) Institute of Technology and Engineering, Massey University, Palmerston North, New Zealand;(2) Fonterra Cooperative Group Ltd., New Zealand |
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Abstract: | Lactobacillus delbrueckii subsp. bulgaricus NCFB 2483, when grown on lactose in continuous culture, showed increasing specific yields and volumetric productivities of exopolysaccharide (EPS) with increasing dilution rate. Specific and volumetric productivities of lactate and galactose, as extracellular metabolites, increased in response to the incremental changes in the dilution rate up to 0.4 h–1. Elevated Yp/s values determined for EPS (0.025 g EPSg lactose–1) at the dilution rates of 0.3 h–1–0.4 h–1, relative to those determined at lower dilution rates, suggest a diversion of carbon flux towards EPS being associated with the higher rates of growth. |
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Keywords: | continuous culture exopolysaccharide growth rate Lactobacillus delbrueckii subsp. bulgaricus |
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