Fluorescent in situ hybridization en suspension (FISHES) using digoxigenin-labeled probes and flow cytometry. |
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Authors: | E A Timm C C Stewart |
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Affiliation: | Department of Flow Cytometry, Roswell Park Cancer Institute, Buffalo, NY 14263. |
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Abstract: | Fluorescent in situ hybridization has become a major technique for visualizing genetic material in fixed cells. Currently, many systems utilize the hybridization of labeled molecular probes to cells that are attached to slides. We have developed a technique that allows for in situ hybridization to be performed using cells in suspension. By using digoxigenin-labeled DNA probes and a fluoresceinated antibody directed against the digoxigenin, we can measure the resulting signal on a flow cytometer and the cells can be attached to microscope slides for visual analysis. |
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