Niemann-Pick Type II fibroblasts exhibit impaired cholesterol esterification in response to shingomyelin hydrolysis |
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Authors: | David M. Byers Matthew W. Morgan Harold W. Cook Frederick B. St. C. Palmer Matthew W. Spence |
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Affiliation: | Atlantic Research Centre for Mental Retardation, Departments of Pediatrics and Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada |
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Abstract: | Fibroblasts from patients with Niemann-Pick Type II disease, including the panethnic type C (NPC) and Nova Scotia Acadian type D (NPD) forms, exhibit reduced or delayed stimulation of cholesterol esterification by low density lipoprotein (LDL). Based on recent evidence that cholesterol esterification can also be stimulated by cell surface sphingomyelin hydrolysis, we have compared the response of normal, NPC and NPD fibroblasts to treatment with exogenous sphingomyelinase (SMase). Staphylococcus aureus SMase (> 0.05 U/ml) hydrolyzed over 90% of endogenous sphingomyelin within 1 h and increased incorporation of [3H]oleic acid into cholesterol-[3H]oleate after an initial lag in all three cell types. However, normal levels of cholesterol esterification were not observed for NP Type II fibroblasts: four NPD cell lines exhibited an average of 32% of normal response while cholesterol esterification was only 20% in two well-characterized NPC lines. A third NPC line exhibited normal response to SMase despite greater than 90% impairment of LDL-stimuated cholesterol esterification. Incubation of fibroblasts with LDL followed by SMase produced a synergistic response, particularly in NPC cells where there was little response to either treatment alone. Chloroquinone abolished LDL-stimulated cholesterol esterification in normal fibroblasts but had no effect on the response to SMase, indicating that lysosomal enzymes may not be involved in SMase-mediated cholesterol esterification. These results suggest that intracellular processing of cholesterol derived from either LDL or release from the plasma membrane (by sphingomyelin hydrolysis) is affected in Niemann-Pick Type II cells and that these pathways can complement one another in the stimulation of cholesterol esterification. |
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Keywords: | Niemann-Pick disease Acyl-CoA cholesterol acyltransferase Sphingomyelinase Human fibroblast ACAT acyl-CoA:cholesterol acyltransferase (EC 2.3.1.26) LDL low density lipoprotein FBS fetal bovine serum LDP-FBS lipoprotein-deficient FBS NPC Niemann-Pick disease type C NPD Niemann-Pick disease type D SMase sphingomyelinase |
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