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Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel electrophoresis analysis |
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| Authors: | Kozue Oana Yoshiyuki Kawakami Tetsuya Hayashi Makoto Ohnishi |
| Affiliation: | Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3-1-1 Asahi, Matsumoto 390-8621;, Division of Bioenvironmental Science, Frontier Science Research Center;and Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, 5200 Kiyotake, Miyazaki 889-1692;and Department of Bacteriology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuka, Tokyo 162-8640, Japan |
| Abstract: | We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols. |
| Keywords: | labiase lytic enzyme pulsed-field gel electrophoresis |