Analysis of an engineered Salmonella flagellar fusion protein, FliR-FlhB |
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Authors: | Van Arnam John S McMurry Jonathan L Kihara May Macnab Robert M |
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Affiliation: | Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA. |
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Abstract: | Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium a fliR-flhB fusion gene exists. We constructed a similar Salmonella fusion gene which is able to complement fliR, flhB, and fliR flhB null strains. Western blotting revealed that the FliR-FlhB fusion protein retains the FlhB protein's cleavage properties. We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio. |
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