Isolation and partial characterization of a novel basement membrane collagen |
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| Authors: | R Dixit M W Harrison S N Dixit |
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| Institution: | 1. Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, China;2. Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China;3. Department of Biomedical Sciences, School of Medicine, Creighton University, Omaha, United States;1. Cellular and Molecular Arrhythmia Research Program, Department of Medicine, University of Wisconsin Madison, WI, USA;2. Department of Cardiology, Beijing Anzhen Hospital, Captial Medical University, National Clinical Research Center for Cardiovascular Diseases, Beijing, China;3. Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA;4. National Heart and Lung Institute, Imperial College London, ICTEM, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK |
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| Abstract: | A guanidine-HCl extraction of lens capsule basement membrane dissolves collagenous material. This material was fractionated on an Agarose A-5M column. Fractions 1, 2 and 3 were further purified and partially characterized immunochemically and by amino acid analysis. Fraction 3 has a molecular weight of 55,000 when compared with collagen type I standard. The CNBr peptide pattern and composition of fraction 3 are different from those of alpha 1 (IV) 95K and alpha 2 (IV) 95K chains. The results described suggest the presence of a new chain in lens capsule basement membrane. |
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