Heterologous expression of a position 2-substituted (1-->3)-beta-D-glucan in Lactococcus lactis |
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Authors: | Werning María Laura Corrales María Angeles Prieto Alicia de Palencia Pilar Fernández Navas Jesús López Paloma |
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Affiliation: | Departamento de Ciencia de Proteínas, Centro de Investigaciones Biológicas, Ramiro de Maeztu 9, 28040 Madrid, Spain. |
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Abstract: | Exopolysaccharides play an important role in the rheology and texture of fermented foods, and among these beta-glucans have immunomodulating properties. We show that the overproduction of the Pediococcus parvulus GTF glycosyltransferase in an uncapsulated Lactococcus lactis strain results in synthesis and secretion (300 mg liter(-1)) of a position 2-substituted (1-->3)-beta-D-glucan that has potential use as a food additive. |
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