Effect of nitrous oxide-induced inactivation of vitamin B12 on glycinamide ribonucleotide transformylase and 5-amino-4-imidazole carboxamide transformylase |
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Authors: | R Deacon J Perry M Lumb I Chanarin |
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Institution: | Department of Biochemistry, University of Aberdeen, Marischal College, Aberdeen AB9 1AS, U.K. |
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Abstract: | Exposure to nitrous oxide (N2O) in vivo is accompanied by oxidation of cobI]-alamin to the inactive cobIII]alamin 1]. There is loss of methionine synthetase activity 2] and evidence of depressed supply of single carbon units at the formate level of oxidation 3,4,5]. We measured the effect of inactivation of B12 on the folate-dependent transformylases concerned in purine synthesis. After 24 h exposure to N2O there was a significant fall in glycinamide ribonucleotide transformylase (EC 2.1.2.2) and a significant increase in 5-amino-4-imidazole carboxamide transformylase (EC 2.1.2.3). |
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Keywords: | HDP helix-destabilising protein |
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