Production of 4,5-dihydro-4,5-dihydroxyphthalate from phthalate by a mutant strain of Pseudomonas testosteroni M4-1 |
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| Authors: | Toshio Omori Minoru Matsubara Susumu Masuda Tohru Kodama |
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| Institution: | (1) Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, 113 Tokyo, Japan;(2) Japan Synthetic Rubber Co., Ltd., Research Center, 25 Miyukigaoka, Tsukuba, 305 Ibaraki, Japan |
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| Abstract: | Summary
Pseudomonas testosteroni M4-1, capable of using phthalate as the sole carbon and energy source, was isolated. Tn5 mutagenesis using pSUP2021 yielded mutant strains of M4-1 that are defective in phthalate metabolism and produce a dihydrodiol compound. The dihydrodiol compound produced by mutant strain M4-122 was isolated and identified as 4,5-dihydro-4,5-dihydroxyphthalate (DDP) by elementary analysis, mass analysis and nuclear magnetic resonance. Various conditions to increase the yield of DDP from phthalate were examined for mutant strain M4-122. With resting cells 6 g DDP/1 were produced. The additional of ethanol to the resting-cell reaction mixture enhanced DDP production and 10 g DDP/1 was produced from 8.3 g/1 of phthalate.
Offprint requests to: T. Omori |
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