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中国普通野生稻核糖体RNA墓因限制性片段长度多态性
引用本文:朱世华,张启发,王明全.中国普通野生稻核糖体RNA墓因限制性片段长度多态性[J].遗传学报,1998(6).
作者姓名:朱世华  张启发  王明全
作者单位:宁波大学师范学院生物系!宁波,315211,华中农业大学作物遗传改良国家重点实验室!武汉,430070,武汉大学生命科学学院遗传学系!武汉,430072
基金项目:国家自然科学基金!38970440
摘    要:对98份普通野生稻、亚洲栽培稻及稻进行了核糖体RNA基因间间隔区的限制性片段长度多态性(RFLP)分析。共发现30种长度变异类型,组成45种表现型。广西普通野生稻的rDNA间隔序列长度多样性最丰富,24份材料中检测到25种长度变异类型,共组成22种表现型。rDNA间隔区长度变异类型的地理分布与纬度相关,高纬度来源的普通野生稻的rDNA间隔区长度较短,与粳稻的长度变异类型相似;低纬度来源的材料rDNA间隔长度趋长,与籼稻的长度变异类型相似;稻的长度变异类型与粳稻相似。提出了华南是野生稻变异中心的观点,并指出对我国野生稻资源进行保护的重要性。

关 键 词:Oryzarufipogon  核糖体RNA基因  基因间间隔序列  遗传多样性

Ribosomal DNA Polymorphisms of the Common Wild Rice from China
ZHU Shi-hua, ZHANG Qi-fa, WANG Ming-quan.Ribosomal DNA Polymorphisms of the Common Wild Rice from China[J].Journal of Genetics and Genomics,1998(6).
Authors:ZHU Shi-hua  ZHANG Qi-fa  WANG Ming-quan
Institution:ZHU Shi-hua; ZHANG Qi-fa; WANG Ming-quan(1 Department of Biology Teacher's College Ningbo University Ningbo 315211)(2 National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070)(3 Department of Genetics College of Bi
Abstract:A sample of 98 accessions of the common wild rice (Oryza rufipogon), Asiancultivated rice and Ludao, mostly from China, were assayed for ribosomal DNApolymorphisms. A total of 30 spacer length variants (slys), which formed 45 rDNAphenotypes, were detected. Wild rice from Guangxi is the richest in diversity; 25 slvswere detected among 24 accessions. The distribution of rDNA spacer length variantswere relaied to latitudes where the samples were collectod. Samples from higherlatitudes showed shorter slys, while those from lower latitudes showed longer ones.Such geographical distributions seem to correspond well with indica-japonicadifferentiation. Ludao is similar to japonica rice in rDNA slvs. The data suggestedthat Southern China is likely to be one of the centers of genetic diversity of wild rice.The results also indicated the importance of conservation and utilization of wild ricegermplasm in China.
Keywords:Oryza rufipogon  rDNA  Intergenic spacer  Genetic diversity
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