Isolated microspore culture and plant regeneration in rye (Secale cereale L.) |
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Authors: | Y-D Guo S Pulli |
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Institution: | (1) Laboratory of Plant Physiology and Molecular Biology, Department of Biology, University of Turku, 20014, Turku, Finland e-mail: yaguo@utu.fi Fax: +358-2-3335549, FI |
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Abstract: | An isolated microspore culture and green plant regeneration method for rye (Secale cereale L.) was established. Rye isolated microspore androgenesis was genotype-dependent. PG-96M medium supplemented with 6% maltose
gave the highest microspore survival rate after 48 h of culture and the highest embryo/callus yield (930 embryos/calli per
100 anthers from cv. Florida 401). Osmotic pressure in the induction medium played an important role. Pretreatment of the
anthers with mannitol was beneficial for the microspore culture. Embryos/calli of a relatively younger age and smaller size
had a higher regeneration ability, with the best green plant regeneration rate being 6%. Over 150 microspore-derived green
plants have been obtained so far. About 90% of the regenerated plants were spontaneous doubled haploids. This is the first
report of isolated microspore culture in true rye resulting in androgenic embryogenesis and plant regeneration.
Received: 26 April 1999 / Accepted: 23 November 1999 |
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Keywords: | Rye Microspore culture Androgenic embryogenesis Genotype Regeneration |
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