Effect of homologous peptides of differentiation factors HLDF and PEDF on preimplantation development of mice in vitro |
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Authors: | Sakharova N Iu Kostanian I A Lepikhova T N Lepikhov K A Malashenko A M Navolotskaia E V Tombran-Tink J Lipkin V M |
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Institution: | Institute of Theoretical and Experimental Biology, Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290 Russia. |
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Abstract: | We studied the effect of synthetic peptides PEDF-6 and HLDF-6 on preimplantation development of mouse embryos in vitro. PEDF-6 peptide corresponds to fragment 351-356 and of pigment epithelium-derived differentiation factor (PEDF), while HLDF-6 peptide corresponds to fragment 84-89 of differentiation factor HLDF isolated from HL-60 cell line. Despite high homology, these peptides had different effects on the early development. PEDF-6 had no effect on the cleavage of 2-4-cell embryos but decelerated blastocyst formation from such embryos and disturbed their structure. In the presence of HLDF-6 the blastomeres divided more actively as compared to the control and a higher number of embryos developed to the blastocyst stage. The effects of both peptides were stage-specific: the affect the embryos at early cleavage stages and, apparently, determine their further development at that moment although do not directly affect formation of the blastocysts. |
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