Research on adducts of benzo(a)pyrene and DNA in different cells using synchronous fluorescence spectrophotometry] |
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| Authors: | A Izzotti R Balansky L Scatolini N Coscia |
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| Institution: | Istituto di Igiene e Medicina Preventiva, Facoltà di Medicina e Chirurgia, Università di Genova. |
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| Abstract: | The levels of benzo(a)pyrene diolepoxide(BPDE)-DNA adducts were measured in rat and human cells by synchronous fluorescence spectrophotometry. BPDE-DNA adducts detected in human pulmonary alveolar macrophages were related to current smoking habits, in contrast to the adducts found in peripheral blood leukocytes. BP administration to rats produced BPDE-DNA adducts in both liver and lungs. Although small yet repeatable signals were also detected in lung DNA from rats treated for 3 days with tobacco smoke. None of the samples obtained from untreated animals was positive. The detection of BPDE-DNA adducts may be used in biomonitoring and experimental studies for determining of exposure to BP even when applied as a constituent of complex mixtures. |
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