花生萌发期耐盐性综合评价及耐盐种质筛选

为了解江苏花生种质资源的耐盐性,挖掘耐盐种质资源,本试验对47份花生种质材料进行耐盐性综合评价,以发芽势、发芽指数、活力指数、发芽率、鲜重、相对含水量、干重的相对值为鉴定指标,采用主成分分析、隶属函数法以及聚类分析方法,对其进行萌发期耐盐性综合评价及耐盐种质筛选。结果表明,花生种质材料萌发期的耐盐性强弱判定结果受多个指标影响,相对含水量和鲜重可以作为花生种质萌发期耐盐性的最佳鉴定指标,5 g/L Na Cl溶液可以作为花生萌发期耐盐性鉴定的合适浓度,47份花生材料划为5个耐盐级别,筛选出JP42、JP29、JP23、JP43、JP35、JP4等6份耐盐性强的种质,JP27和JP98为高度敏感材料,隶属函数法结合耐盐分级可以作为一种简便快速鉴定花生萌发期耐盐性的方法。

Comprehensive Evaluation of Salt Tolerance and Screening for Salt Tolerant Accessions of Peanut at Germination Stage

In order to know the peanut germplasm resources in Jiangsu and explore salt tolerant resource. In this experiment, 47 peanut germplasm materials were treated with salt stress with 5 g/L NaCl, and the principal component analysis, membership function and cluster analysis were used based on the relative value of germination rate, germination energy, germination index, vigor index, fresh weight , relative water content , dry weight . The results showed that salt tolerance evaluation of peanut lines was affected with multiple indexes, relative water content and fresh weight could be used as the evaluation indexes of salt tolerance in peanut germplasm, and 5 g/L NaCl solution could be used as the suitable concentration of salt tolerance in peanut germination. 47 peanut materials were divided into 5 levels of salt tolerance, and JP42, JP29, JP23, JP43, JP35, JP4, and so on were screened out for the 6 salt tolerant germplasm. JP27 and JP98 are highly sensitive materials, and the method of membership function combined with salt tolerance can be used as a simple and rapid method for the identification of salt tolerance at germination stage.