Hsp 27 is better associated with the expression of inducible thermotolerance in human pancreatic tumor cell lines than hsp 70, p53 or p21/waf1/cip1 |
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| Institution: | 1. Ottawa Regional Cancer Centre, 503 Smyth Road, Ottawa, Ont., Canada K1H 1C4;2. Department of Cellular and Molecular Medicine, University of Ottawa, 451 Smyth Road, Ottawa, Ont., Canada K1H 8M5;3. Department of Biology, University of Ottawa, Gendron Hall, 30 Marie Curie St., Ottawa, Ont., Canada K1N 6N5;1. School of Management, Hefei University of Technology, Hefei, Anhui 230009, China;2. DeGroote School of Business, McMaster University, Hamilton, Ontario L8S 4M4, Canada;3. John Molson School of Business, Concordia University, Montreal H3H 1J5, Canada;1. Institute for Process and Particle Engineering, Graz University of Technology, Inffeldgasse 13, 8010 Graz, Austria;2. SRL, Office of Technology 2-44, Western Gateway Building, University College Cork, Ireland;3. Department of Food and Drug, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy;4. Drug Product Design, Pharmaceutical Sciences, Pfizer, Discovery Park House, Ramsgate Road, Sandwich, Kent CT13 9NJ, UK;5. Research Center Pharmaceutical Engineering GmbH, Inffeldgasse 13, 8010 Graz, Austria;6. SSPC Pharmaceutical Research Centre, School of Pharmacy, University College Cork, Cork, Ireland;7. ClinSearch, 110 Avenue, Pierre Brossolette, 92240 Malakoff, France;8. CMAC Technology and Innovation Centre, University of Strathclyde, 99 George Street, Glasgow G1 1RD, UK;9. Department of Clinical, Pharmaceutical and Biological Sciences, University of Hertfordshire, College Lane, Hatfield AL10 9AB, UK;10. School of Pharmacy, Aston University, Birmingham, B4 7ET, UK;11. Welfenstrasse 14, D-55218 Ingelheim, Germany;12. Ecole de Biologie Industrielle, 49 avenue des Genottes, 95895 Cergy Cedex, France;1. Department of Aeronautical Engineering, Vel Tech Dr. Rangarajan Dr. Sagunthala R&D Institute of Science & Technology, Chennai, India;2. Department of Mechanical Engineering, Saveetha School of Engineering, SIMATS, Chennai, India;3. Department of Electronics and Communication Engineering, Sri Sai Ram Institute of Technology, Chennai, Tamilnadu, India;1. School of Statistics, Dongbei University of Finance and Economics, Dalian 116025, China;2. Postdoctoral Research Station of Applied Economics, Zhejiang University of Finance and Economics, Hangzhou 310018, China;3. Institute of Systems Engineering, Macau University of Science and Technology, Taipa Street, Macau;1. School of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia;2. Institute of Microengineering and Nanoelectronics (IMEN), Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia;3. Department of Flexible and Printable Electronics, Chonbuk National University, Jeonju 561-756, Republic of Korea;4. Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia |
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| Abstract: | We evaluated the levels of p53, p21/waf1/cip1, hsp 27 and hsp 70 proteins in confluent cultures of human pancreatic tumor cell lines during the expression of inducible thermotolerance (IT). Two of these cell lines have wild type, whereas the other two have mutant, p53 status. Three cell lines expressed a significant amount of IT (p<0.05 and better, one-tailed Student's t-test); the fourth manifested little IT. Hsp 70 was upregulated in all cell lines. In contrast, hsp 27 was upregulated only in the cell lines that expressed IT. There was no correlation between either p53, or p21, protein levels and propensity to express IT. We conclude that hsp 27 is better associated with the expression of IT than hsp 70 in these cell lines. |
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