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Thermodynamics of single strand DNA base stacking |
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| Authors: | Ramprakash Jayanthi Lang Brian Schwarz Frederick P |
| Affiliation: | Center for Advanced Research in Biotechnology, National Institute of Standards and Technology, 9600 Gudelsky Drive, Rockville, MD 20850. |
| Abstract: | The thermodynamics of the stacking to unstacking transitions of 24 single-stranded DNA sequences (ssDNA), 10-12 bases in length, in sodium phosphate buffer were determined from 10 to 95 degrees C, using differential scanning calorimetry (DSC). An additional 22 ssDNA sequences did not exhibit an S<-->U transition in this temperature range. The transition properties of the ssDNA sequences with |
| Keywords: | differential scanning calorimetry single strand DNA stacking transitions thermodynamics transition enthalpy transition temperature |
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