STUDIES ON NITELLA HAVING ARTIFICIAL CELL SAP I. REPLACEMENT OF THE CELL SAP WITH ARTIFICIAL SOLUTIONS |
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Authors: | TAZAWA MASASHI |
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Institution: | Department of Biology, Faculty of Science, Osaka University Nakanoshima Osaka |
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Abstract: | A method for replacing the cell sap of Nitella with an artificialsolution was introduced. The technique, which is a modificationof KAMIYA and KURODA'S (1, 2), is applicable not only for isotonicbut also for hypertonic or hypotonic solutions. Photometricdeterminations of K+, Na+, Ca++ and Cl proved that thereplacement of the cell sap with the present method is satisfactory.The internodal cell of Nitella, whose cell sap was replacedwith an isotonic solution with a simple composition such asa mixture of KCl, NaCl and CaCl2, can be kept living at leastfor several days, sometimes even for more than one month. (Received September 6, 1963; ) |
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