Large dense-core vesicle exocytosis in pancreatic β-cells monitored by capacitance measurements |
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| Authors: | Takahiro Kanno, Xiasong Ma, Sebastian Barg, Lena Eliasson, Juris Galvanovskis, Sven G pel, Max Larsson, Erik Renstr m,Patrik Rorsman |
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| Affiliation: | a Department of Physiology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan;b Department of Physiological Sciences, Lund University, BMC B11, SE-221 84, Lund, Sweden |
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| Abstract: | This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic β-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other. |
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