| Abstract: | Recombinant cDNA libraries were constructed from poly(A)+ RNA isolated from different stages of oogenesis and embryogenesis from the clawed toad Xenopus laevis. Hybridization analyses were used to describe the accumulation of specific RNAs represented by these cDNA clones in oocytes, embryos, adult liver, a cell line derived from Xenopus borealis embryos (Xb693), and a tumorigenic substrain of that cell line (Xb693T). It was found that from 550 cDNA clones analysed, six sequences accumulate to higher titers in poly(A)+ RNA isolated from the tumorigenic cell line compared with the non-tumorigenic cell line. All six sequences were expressed at high levels during oogenesis, and the titers of three of these sequences decreased considerably during oogenesis. DNA sequencing of these three sequences followed by a computer search of protein data banks has identified them as coding for the glycolytic enzyme enolase, the ATP-ADP carrier protein, and a-tubulin. |
