椰子织蛾幼虫肠道细菌的初步分离鉴定及功能分析

目的] 研究椰子织蛾幼虫肠道微生物的种类和功能，以揭示其消化利用寄主老叶的机制。方法] 采用传统微生物分离培养技术分离培养肠道细菌，用16S rRNA基因序列分析的方法鉴定菌株，采用透明圈染色法对所得菌株进行功能性验证。结果] 基因序列检测对比鉴定得到9种可培养细菌菌株，主要属于变形菌门和厚壁菌门以及放线菌门；功能性验证结果表明，伯克霍尔德氏菌、解淀粉芽孢杆菌、贝莱斯芽孢杆菌、蜡样芽孢杆菌菌株具有纤维素降解酶，寒气玫瑰单胞菌、解淀粉芽孢杆菌含木聚糖降解酶。结论] 椰子织蛾肠道中存在可培养的具有降解纤维素及木聚糖能力的细菌，这些细菌可能有助于椰子织蛾取食消化椰子等老叶，研究所获得的肠道微生物菌株也为后续研究该虫与环境的关系及相关菌株应用于农业、能源、环保价值的探索提供帮助。

Preliminary identification and functional analysis of larval gut bacteria in Opisina arenosella Walker

Aim] The gut microbes of insects have an important influence on host selection. In this paper, the species and functions of gut microorganisms in the larvae of Opisina arenosella were studied in order to reveal the digestion mechanism when eating old leaves of coconut.Method] Traditional microbial isolation and culture technology were used to isolate and culture intestinal bacteria, and then 16S rRNA gene sequence analysis was performed to identify the species, and then transparent circle staining was used to verify the functions of the strains.Result] Nine culturable bacterial strains were identified by gene sequence detection, mainly Proteobacteria, Phylum Firmicutes and Actinobacteria. The results of functional verification showed that Burkholderia lata, Bacillus amyloliquefaciens, Bacillus velezensis and Bacillus cereus had cellulolytic enzymes. Roseomonas aerofrigidensis and Bacillus amyloliquefaciens contained xylan-degrading enzymes.Conclusion] There were culturable bacteria capable of degrading cellulose and xylan in the gut of this insect species. These bacteria may help the moth feed on old leaves such as coconut. The gut microbial strains obtained from the research also provide insights for future studies on the relationship between the insect and its environment, and the application of related strains in agriculture, energy and environmental protection.