Drosophila melanogaster model for Mycobacterium abscessus infection |
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| Affiliation: | 1. Drug Biology, Institut Pasteur Korea, Seongnam-si, Gyeonggi-do, Republic of Korea;2. Antibacterial Drug Discovery, Institut Pasteur Korea, Seongnam-si, Gyeonggi-do, Republic of Korea;3. Department of Clinical Laboratory Science, Semyung University, Jecheon, Chungbuk, Republic of Korea;4. Chuncheon Center, Korea Basic Science Institute, Chuncheon, Gangwon-do, Republic of Korea;1. Department of Biochemistry, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Jehangir Merwanji Street, Parel, Mumbai 400012, Maharashtra, India;2. Molecular and Cellular Biology Laboratory, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Jehangir Merwanji Street, Parel, Mumbai 400012, Maharashtra, India;1. Department of Biochemistry, Center of Biological Sciences, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis, SC, Brazil;2. Post-Graduate Nutrition Program, Center of Health Sciences, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis, SC, Brazil;1. Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 1401 Rockville Pike, HFM-431, Rockville, MD 20852, USA;2. Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada |
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| Abstract: | Mycobacterium abscessus is a human pathogen that is responsible for a broad spectrum of tissue infections and disseminated infections in immunodeficient patients. This pathogen is one of the most resistant organisms to chemotherapeutic agents. Therefore, we tested the hypothesis that the fruit fly, Drosophila melanogaster, is a genetically tractable model host for M. abscessus. In this context, we infected D. melanogaster with M. abscessus. This M. abscessus infection results in dissemination in the fly body, followed by death, which is accompanied by severe indirect flight muscle and brain damage. Our data show that M. abscessus can grow and replicate in D. melanogaster w1118 and that it elicited a humoral immune response, especially of the Toll antimicrobial peptide pathway. To the best of our knowledge, this is the first report that mycobacteria induce the production of antimicrobial peptides in D. melanogaster. |
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| Keywords: | Invertebrate model host Antimicrobial peptide |
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