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Photocrosslinking approaches to interactome mapping
Institution:1. Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK;1. Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea;2. Division of Mechanical Engineering for Creative Emerging Technologie, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea;3. Medical Research Institute, School of Medicine, Pusan National University, Yangsan 626-770, Republic of Korea;4. Laboratory for Vascular Medicine and Stem Cell Biology, Department of Physiology, School of Medicine, Pusan National University, Yangsan 626-770, Republic of Korea;5. Convergence Stem Cell Research Center, Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Pusan National University, Yangsan 626-770, Republic of Korea
Abstract:
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  • Highlights► Photocrosslinking provides insight into context-dependent interactions. ► Interactome dynamics can be determined by photocrosslinking. ► Use of photocrosslinkers defines specific interaction interfaces within large complexes. ► Crosslinking captures transient interaction complexes containing IDPs and glycoproteins.
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