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Pathway and protein engineering approaches to produce novel and commodity small molecules
Institution:1. Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, United States;2. Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, United States;1. VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland;2. Global Technology, SK Innovation, Daejeon, Republic of Korea;1. Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, 94608, United States;2. State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, School of Life Sciences, Hubei University, Wuhan, Hubei, 430062, PR China;3. Advanced Biofuels & Bioproducts Process Development Unit, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, United States;4. Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, United States;5. Department of Chemical & Biomolecular Engineering, University of California, Berkeley, CA, 94720, United States;6. School of Public Health, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, PR China;7. Novo Nordisk Foundation Center for Biosustainability, Technical University Denmark, DK 2970 Horsholm, Denmark;8. Center for Synthetic Biochemistry, Shenzhen Institutes for Advanced Technologies, Shenzhen, Guangdong, 518055, PR China;1. Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA;2. Asahi Kasei Corporation, 2-1 Samejima, Fuji, Shizuoka 416-8501, Japan
Abstract:
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  • Highlights? Production of novel pharmaceutical and commodity chemicals. ? Protein and pathway engineering pave the way for product diversifications. ? ‘Omics’ data have helped assembly of pathways from different species. ? Recombination of enzymatic domains and site-directed mutagenesis. ? Tunable control of pathways for better production.
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